Open Access<p>Evaluation Of The Efficacy Of Fluorescent Staining And Chicago Sky Blue Staining As Methods For Diagnosis Of Dermatophytosis In Hair And Nails</p>
Author(s) -
Basma Mourad,
Mayada Ismail,
Soha Abdalla Hawwam,
Mina Msseha,
Reham Hassan
Publication year - 2019
Publication title -
clinical, cosmetic and investigational dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 35
ISSN - 1178-7015
DOI - 10.2147/ccid.s215661
Subject(s) - staining , nail (fastener) , stain , fluorescent staining , agar , dermatology , microbiology and biotechnology , pathology , medicine , biology , materials science , bacteria , metallurgy , genetics
Dermatophytes are fungi that cause infections affecting hair, nail, and skin; in nails they cause onychomycosis, while in hair they lead to tinea capitis. Detection of dermatophytes using traditional methods, including potassium hydroxide (KOH) and culture on agar-based media leads to high rates of false-negative results. Here, we investigated more accurate diagnostic techniques, including Chicago sky blue staining and Calcofluor white fluorescent staining and compared them with traditional KOH and culture methods for the diagnosis of fungi causing onychomycosis and tinea capitis.