Open AccessDevelopment of multiplexed reverse-transcription loop-mediated isothermal amplification for detection of SARS-CoV-2 and influenza viral RNA
Author(s) -
Yinhua Zhang,
Nathan A. Tanner
Publication year - 2021
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2020-0157
Subject(s) - reverse transcription loop mediated isothermal amplification , loop mediated isothermal amplification , virology , multiplex , reverse transcriptase , multiplexing , rna , pandemic , influenza a virus , transcription (linguistics) , biology , covid-19 , computational biology , virus , medicine , computer science , gene , bioinformatics , genetics , dna , telecommunications , linguistics , disease , philosophy , pathology , infectious disease (medical specialty)
The ongoing pandemic has demonstrated the utility of widespread surveillance and diagnostic detection of the novel SARS-CoV-2. Reverse-transcription loop-mediated isothermal amplification (RT-LAMP) has enabled broader testing, but current LAMP tests only detect single targets and require separate reactions for controls. With flu season in the Northern Hemisphere, the ability to screen for multiple targets will be increasingly important, and the ability to include internal controls in RT-LAMP allows for improved efficiency. Here we describe multiplexed RT-LAMP with four targets (SARS-CoV-2, influenza A, influenza B, human RNA) in a single reaction using real-time and end point fluorescence detection. Such increased functionality of RT-LAMP will enable even broader adoption of this molecular testing approach and aid in the fight against this public health threat.