Open AccessInjection of Evans blue dye to fluorescently label and image intact vasculature
Author(s) -
Samuel Honeycutt,
Lori L. O’Brien
Publication year - 2021
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2020-0152
Subject(s) - evans blue , fluorescence , fluorescence microscope , biomedical engineering , microscopy , fluorescent labelling , visualization , chemistry , pathology , computer science , artificial intelligence , medicine , optics , physics
Blood vessels perform critical functions in both health and disease. Understanding how vessels form, pattern and respond to damage is essential. However, labeling and imaging the vasculature to ascertain these properties can be difficult and time-consuming. Here, the authors present a novel methodology for rapidly and efficiently labeling whole vascular networks in vivo by exploiting the fluorescent properties of Evans blue. By combining the labeling with fluorescence microscopy, this method enables visualization of whole tissue vasculature for a fraction of the time and cost compared with traditional methods.