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Evaluation of the Desiccation of Campomanesia adamantium Seed Using Radiographic Analysis and the Relation with Physiological Potential
Author(s) -
LeãoAraújo Érica F.,
Gomes Francisco G.,
Silva Anderson R.,
Peixoto Nei,
Souza Eli Regina B.
Publication year - 2019
Publication title -
agronomy journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 131
eISSN - 1435-0645
pISSN - 0002-1962
DOI - 10.2134/agronj2018.05.0302
Subject(s) - germination , desiccation , seedling , dehydration , horticulture , biology , water content , recalcitrant seed , agronomy , botany , biochemistry , geotechnical engineering , engineering
Core Ideas Dehydration of C. adamantium seed is followed by a decrease in seed viability and vigor. Physical injury damage and internal free space increase as the seed dehydrates. X‐ray images can be used to evaluate the internal morphology of C. adamantium seed. Internal morphology is related to germination and vigor.Sensitivity to desiccation is a characteristic that hinders the ex situ conservation of seeds, which can result in reduction of viability and vigor as the water is lost. Dehydration is sometimes followed by the occurrence of injuries and retraction of tissues. In this study, radiographic images of Campomanesia adamantium (Cambess.) O. Berg. seed were used to evaluate changes caused by desiccation in the internal morphology and their relationships with germination and vigor. The initial water content of the seed was determined (48%) and adjustments were made to obtain samples with water content of 36, 30, 27, 24, and 21% (wet basis). Evaluations of germination, germination first count, speed germination index, mean germination time, and seedling length were performed. Individual radiographs of the seed were obtained using digital x‐ray equipment. The images were used to identify cracked seed (CS) and to determine the internal free space (IFS). Data were subjected to analysis of variance and the effect of desiccation studied through polynomial regression. Seed viability and vigor decreased linearly as the seed desiccated. The results indicated that IFS and CS are positively associated with the loss of water. The loss of germination and vigor of C. adamantium seed is related to changes in the internal morphology during desiccation, which can be identified by x‐ray imaging.