Open AccessIDENTIFIKASI E. COLI O157:H7, SALMONELLA SP, DAN SENSITIFITAS ANTIBIOTIKA DARI SUSU KAMBING DAN PRODUK OLAHANNYA
Author(s) -
Widodo Suwito,
nFN Andriani,
Felisitas Kristiyanti,
Erna Winarti
Publication year - 2019
Publication title -
jurnal penelitian pascapanen pertanian/jurnal penelitian pascapanen pertanian
Language(s) - English
Resource type - Journals
eISSN - 2541-4054
pISSN - 0216-1192
DOI - 10.21082/jpasca.v15n1.2018.36-42
Subject(s) - escherichia coli , salmonella , chemistry , microbiology and biotechnology , biology , bacteria , biochemistry , gene , genetics
Susu kambing dan produk olahannya dapat terkontaminasi E. coli O157:H7 dan Salmonella sp. Tujuan penelitian ini untuk isolasi dan identifikasi E. coli O157:H7, Salmonella sp, dan sensitifitasnya terhadap antibiotika dari susu kambing dan produk olahannya. Sebanyak 15 sampel susu kambing dan produk olahannya seperti susu bubuk, permen, es krim, yogurt, dan krupuk masing-masing sebanyak 10, 3, 6, 4, dan 3 sampel. Semua sampel diperiksa terhadap E. coli O157:H7, dan Salmonella sp berdasarkan reaksi biokimia. Jumlah E. coli pada semua sampel dihitung dengan most probable number (MPN), sedangkan sensitifitas terhadap antibiotika dengan agar difusi. Hasil penelitian menunjukkan bahwa susu kambing dan produk olahannya tidak ditemukan E. coli O157:H7 dan Salmonella sp., tetapi E. coli non O157:H7 ditemukan pada susu kambing sebanyak 3/15 (20%) dengan jumlah >6 MPN/100ml. Semua produk olahan susu kambing memiliki jumlah E. coli <3 MPN/100ml. E. coli dari susu kambing resisten terhadap cefiksime, kanamisin, tetrasiklin, sulfonamide, dan oksitetrasiklin masing-masing sebanyak 1/3 (30%), sedangkan ampisilin dan amoksilin 100%. Goat milk and dairy products could be contaminated with E. coli O157:H7 and Salmonella sp . The purpose of this study was to isolation and identification of E. coli O157:H7, Salmonella sp, and antibiotic sensitivity from goat milk and dairy products. A total of 15 samples from goat milk and dairy products such as milk powder, candy, ice cream, yogurt, and crackers respectively 10, 3, 6, 4, and 3 samples. All samples were analyzed for E. coli O157: H7, and Salmonella sp with biochemical reaction. Total of E. coli in all samples was measured with most probable number (MPN) and antibiotic sensitivity with diffusion agar. These study showed that goat milk and dairy products not found E. coli O157:H7 and Salmonella sp., whereas E. coli non O157:H7 was found in goat milk 3/15 (20%) with total E. coli >6 MPN/100ml. All dairy goat products have total E. coli <3 MPN/100ml. E. coli from goat milk was resistant to cefixime, kanamycin, tetracycline, sulfonamide, and oxytetracycline 1/3 (30%) respectively, but ampicillin and amoxicillin 100%.