Open AccessComparison among plaque assay, tissue culture infectious dose (TCID ) and real-time RT-PCR for SARS-CoV-2 variants quantification
Author(s) -
María I. Zapata-Cardona,
Lizdany Flórez-Álvarez,
Diana Maryory Gómez-Gallego,
María Juliana Moncada-Díaz,
Juan Carlos Hernández,
Francisco J. Díaz,
Marı́a Teresa Rugeles,
Wbeimar Aguilar-Jiménez,
Wildeman Zapata
Publication year - 2022
Publication title -
iranian journal of microbiology.
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.37
H-Index - 27
eISSN - 2008-4447
pISSN - 2008-3289
DOI - 10.18502/ijm.v14i3.9758
Subject(s) - titer , virology , virus quantification , real time polymerase chain reaction , biology , infectious dose , viral load , plaque forming unit , virulence , covid-19 , virus , microbiology and biotechnology , infectious disease (medical specialty) , medicine , gene , pathology , disease , genetics
SARS-CoV-2 variants of concern (VOC) and interest (VOI) pose a significant threat to public health because the rapid change in the SARS-CoV-2 genome can alter viral phenotypes such as virulence, transmissibility and the ability to evade the host response. Hence, SARS-CoV-2 quantification techniques are essential for timely diagnosis and follow-up. Besides, they are vital to understanding viral pathogenesis, antiviral evaluation, and vaccine development.