Open AccessAnalysis of carbapenemases genes of carbapenem-resistant Klebsiella pneumoniae isolated from Tehran heart center
Author(s) -
Leyla Pourgholi,
Hadi Farhadinia,
Maryam Hosseindokht,
Shayan Ziaee,
Rahim Nosrati,
Marjan Nosrati,
Mohammadali Boroumand
Publication year - 2022
Publication title -
iranian journal of microbiology.
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.37
H-Index - 27
eISSN - 2008-4447
pISSN - 2008-3289
DOI - 10.18502/ijm.v14i1.8799
Subject(s) - imipenem , microbiology and biotechnology , klebsiella pneumoniae , amikacin , cefoxitin , cefotaxime , aztreonam , meropenem , cefepime , biology , ciprofloxacin , carbapenem , ceftazidime , ertapenem , antibiotic resistance , antibiotics , gene , pseudomonas aeruginosa , bacteria , escherichia coli , genetics , staphylococcus aureus
Background and Objectives: Emerging of carbapenem-resistant Klebsiella pneumoniae (CRKP) is one of the major con- cerns among healthcare systems. This study aimed to investigate the antibiotic susceptibility pattern and carbapenemase genes of carbapenemase-producing K. pneumoniae isolates obtained from Iranian hospitalized patients.
Materials and Methods: This study was performed on 71 CRKP strains isolated from different clinical specimens collected in Tehran Heart Center (Tehran, Iran). A Modified Hodge test (MHT) was done for the detection of carbapenemase-producing K. pneumoniae. The presence of bla method. KPC , bla VIM , bla IMP , bla , and bla OXA-48 -type carbapenemases was evaluated by the PCR
Results: We identified 8.82% (71/805) of K. pneumoniae isolates as CRKP by MHT test. The antibiotic susceptibility indi- cated that all isolates were resistant to imipenem, meropenem, cefotaxime, ceftazidime, cefepime, ceftriaxone, cephalothin, ciprofloxacin, and augmentin, and then mostly resistant to aztreonam, cefoxitin, gentamicin, and trimethoprim/sulfamethox- azole with 98.6%, 98.6%, 97.2%, and 94.4%, respectively. The lowest resistance was related to amikacin with 46.5% (33/71 isolates). The level of imipenem MIC for all carbapenem-resistant isolates was estimated ≥32 µg/mL. Among positive iso- lates for carbapenemase genes, the most frequent gene was bla . It was found in 48 (67.6%) isolates followed by bla VIM in 28 (39.4%) isolates. bla IMP, bla , and bla KPC genes were identified in 19 (26.8%), 13 (18.3%) and 5 (7.0%) isolates, respectively. These genes were not detected in nine isolates.
Conclusion: The relatively high frequency of some carbapenemase genes suggests major concern about the emergence of isolates containing carbapenem resistance genes as a potential health threat.