Open AccessThe Effects of Combined SarCNU and Ganglioside Treatment of Growth of C6 Glioma Cell Cultures
Author(s) -
Adlija Jevrić-Čaušević
Publication year - 1998
Publication title -
bosnian journal of basic medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 25
eISSN - 1840-4812
pISSN - 1512-8601
DOI - 10.17305/bjbms.1998.3608
Subject(s) - ganglioside , cytotoxicity , glioma , mtt assay , cell culture , in vitro , pharmacology , chemistry , cell , cell growth , neuroprotection , microbiology and biotechnology , biology , biochemistry , cancer research , genetics
In vitro antiproliferative effects of SarCNU and GMI ganglioside were tested in different concentration ratios in C6 glioma cell culture. Based on MTT assay and a protein assay, cytotoxicity of each agent separately and the two agents combined was tested. When the cells were treated with different concentrations of ganglioside ranging from 10-’ to 10-2, the number of viable cells decreased highly on days two and seven, regardless of the concentration of the agent applied. SarCNU, as expected, has caused antiproliferative effects which highly correlated with the concentration of the agent tested. When the C6 glioma cell culture was treated with the two agents combined in the optimal concentration causing the above mentioned cytotoxicity, effects observed did not correlate with those observed for each agent alone, suggesting in this case the expression of highly neuroprotective effects of ganglioside GMI.