Open AccessHuman Umbilical Cord Blood Cells Differentiate into Muscle in sjl Muscular Dystrophy Mice
Author(s) -
Kong Kimi Y.,
Ren Jingmei,
Kraus Morey,
Finklestein Seth P.,
Brown Robert H.
Publication year - 2004
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.22-6-981
Subject(s) - biology , dysferlin , cd34 , stem cell , muscular dystrophy , umbilical cord , transplantation , progenitor cell , limb girdle muscular dystrophy , myf5 , immunology , myocyte , pathology , myogenesis , microbiology and biotechnology , medicine , myogenin , mutation , genetics , gene
Abstract Limb girdle muscular dystrophy type 2B form (LGMD‐2B) and Miyoshi myopathy (MM) are both caused by mutations in the dysferlin ( dysf ) gene. In this study, we used dysferlin‐deficient sjl mice as a mouse model to study cell therapy for LGMD‐2B and MM. A single‐blind study evaluated the therapeutic potential of human umbilical cord blood (HUCB) as a source of myogenic progenitor stem cells. Three groups of donor cells were used: unfractionated mononuclear HUCB cells, HUCB subfractionated to enrich for cells that were negative for lineage surface markers (LIN − ) and substantially enriched for the CD34 surface marker (CD34 + ), and irradiated control spleen cells. We administrated 1 × 10 6 donor cells to each animal intravenously and euthanized them at different time points (1–12 weeks) after transplantation. All animals were immunosuppressed (FK506 and leflunomide) from the day before the injection until the time of euthanasia. Immunohistochemical analyses documented that a small number of human cells from the whole HUCB and LIN − CD34 +/− ‐enriched HUCB subgroups engraft in the recipient muscle to express both dysferlin and human‐specific dystrophin at 12 weeks after transplantation. We conclude that myogenic progenitor cells are present in the HUCB, that they can disseminate into muscle after intravenous administration, and that they are capable of myogenic differentiation in host muscle.