English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Human embryonic stem cells (hESCs) have been derived from the inner cell mass (ICM) of day 5–7 blastocysts and hold great promise for research into human developmental biology and the development of cell therapies for the treatment of human diseases. We report here that our novel three‐step culture conditions successfully support the development of day‐8 human blastocysts, which possess significantly ( p  &lt;.01) more ICM cells than day‐6 blastocysts. Plating of ICMs isolated from day‐8 blastocysts resulted in the formation of a colony with hESC morphology from which a new hESC line (hES‐NCL1) was derived. Our stem cell line is characterized by the expression of specific cell surface and gene markers: GTCM‐2, TG343, TRA1‐60, SSEA‐4, alkaline phosphatase,  OCT‐4 ,  NANOG , and  REX‐1 . Cytogenetic analysis of the hESCs revealed that hES‐NCL1 line has a normal female (46, XX) karyotype. The pluripotency of the cell line was confirmed by the formation of teratomas after injection into severely combined immunodeficient mice and spontaneous differentiation under in vitro conditions.

Derivation of Human Embryonic Stem Cells from Day‐8 Blastocysts Recovered after Three‐Step In Vitro Culture