Open AccessComparison of Various Bone Marrow Fractions in the Ability to Participate in Vascular Remodeling After Mechanical Injury
Author(s) -
Sahara Makoto,
Sata Masataka,
Matsuzaki Yumi,
Tanaka Kimie,
Morita Toshihiro,
Hirata Yasunobu,
Okano Hideyuki,
Nagai Ryozo
Publication year - 2005
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2005-0012
Subject(s) - cd34 , bone marrow , biology , transdifferentiation , haematopoiesis , stem cell , hematopoietic stem cell , microbiology and biotechnology , green fluorescent protein , immunology , biochemistry , gene
Abstract In contrast to conventional assumption, recent reports propose the possibility that hematopoietic stem cells (HSCs) may have broader potential to differentiate into various cell types. Here, we tested the pluripotency of HSCs by comparing vascular lesions induced by mechanical injury after bone marrow reconstitution with total bone marrow (TBM) cells, c‐Kit + Sca‐1 + Lin − (KSL) cells, or a single HSC cell (Tip‐SP CD34 − KSL cell, CD34 − c‐Kit + Sca‐1 + Lin − cell with the strongest dye‐efflux activity) harboring green fluorescent protein (GFP). The lesions contained a significant number of GFP‐positive cells in the TBM and KSL groups, whereas GFP‐positive cells were rarely detected in the HSC group. These results suggest that transdifferentiation of a highly purified HSC seems to be a rare event, if it occurs at all, whereas bone marrow cells including the KSL fraction can give rise to vascular cells that substantially contribute to repair or lesion formation after mechanical injury.