Open AccessCharacterization of Ionic Currents in Human Mesenchymal Stem Cells from Bone Marrow
Author(s) -
Li GuiRong,
Sun Haiying,
Deng Xiuling,
Lau ChuPak
Publication year - 2005
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2004-0213
Subject(s) - iberiotoxin , biology , ion channel , patch clamp , mesenchymal stem cell , tetrodotoxin , biophysics , bone marrow , microbiology and biotechnology , electrophysiology , potassium channel , biochemistry , immunology , neuroscience , receptor
This study characterized functional ion channels in cultured undifferentiated human mesenchymal stem cells (hMSCs) from bone marrow with whole‐cell patch clamp and reverse transcription polymerase chain reaction (RT‐PCR) techniques. Three types of outward currents were found in hMSCs, including a noise‐like rapidly activating outward current inhibited by the large conductance Ca 2+ ‐activated K + channel (I KCa ) blocker iberiotoxin, a transient outward K + current (I to ) suppressed by 4‐aminopyridine (4‐AP), and a delayed rectifier K + current (IK DR )‐like ether‐à‐go‐go (eag) K + channel. In addition, tetrodotoxin‐sensitive sodium current (I Na.TTX ) and nifedipine‐sensitive L‐type Ca 2+ current (I Ca.L ) were also detected in 29% and 15% hMSCs, respectively. Moreover, RT‐PCR revealed the molecular evidence of high levels of mRNA for the functional ionic currents, including human MaxiK for I KCa , Kv4.2 and Kv1.4 for I to , heag1 for IK DR , hNE‐Na for I Na.TTX , and CACNAIC for I Ca.L . These results demonstrate that multiple functional ion channel currents—that is, I KCa , I to , heag1, I Na.TTX , and I Ca.L —are expressed in hMSCs from bone marrow.