Open AccessSimultaneous Injection of Bone Marrow Cells and Stromal Cells into Bone Marrow Accelerates Hematopoiesis In Vivo
Author(s) -
Zhang Yuming,
Adachi Yasushi,
Suzuki Yasuhiro,
Minamino Keizo,
Iwasaki Masayoshi,
Hisha Hiroko,
Song Chang Ye,
Kusafuka Kimihide,
Nakano Keiji,
Koike Yasushi,
Wang Jianfeng,
Koh Eitaku,
Cui Yunze,
Li Chunfu,
Ikehara Susumu
Publication year - 2004
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2004-0173
Subject(s) - stromal cell , bone marrow , haematopoiesis , biology , stem cell , in vivo , immunology , cancer research , microbiology and biotechnology
We have previously demonstrated that stromal cells can support the proliferation and differentiation of hematopoietic cells in vitro and in vivo and that a major histocompatibility complex restriction exists between hematopoietic stem cells and stromal cells. We have also found that intra–bone marrow (IBM) injection of allogeneic bone marrow cells (BMCs) leads to more rapid reconstitution of hematopoietic cells than intravenous injection. In the present study, we examine the effect of simultaneous injection of stromal cells and BMCs into the same bone marrow on the recovery of donor hematopoietic cells and demonstrate that simultaneous IBM injection of BMCs plus stromal cells is more effective in reconstituting recipients with donor hematopoietic cells than intravenous injection of BMCs plus stromal cells or IBM injection of BMCs alone.