z-logo
open-access-imgOpen Access
An Autogeneic Feeder Cell System That Efficiently Supports Growth of Undifferentiated Human Embryonic Stem Cells
Author(s) -
Stojkovic Petra,
Lako Majlinda,
Stewart Rebecca,
Przyborski Stefan,
Armstrong Lyle,
Evans Jerome,
Murdoch Alison,
Strachan Tom,
Stojkovic Miodrag
Publication year - 2005
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2004-0137
Subject(s) - biology , embryonic stem cell , stem cell , microbiology and biotechnology , genetics , gene
Human embryonic stem cells (hESCs) have great potential as a source of cells for therapeutic uses, but their culture requires the support of mouse or human cells, either directly as a feeder cell layer or indirectly as a source of conditioned medium in feeder‐free culture systems. Unfortunately, the risks of cross‐transfer of pathogens from xenogeneic or allogeneic feeders or cell by‐products limit their medical applications. In addition, not all human feeders support the growth of hESCs equally well, and ethical concerns have been raised regarding the derivation of feeder cells from aborted human fetuses. We report here the culture of hESCs on a novel feeder cell system, comprising fibroblast‐like cells derived from the spontaneous differentiation of hESCs. Isogenicity of the hESCs and hESC‐derived fibroblasts was confirmed by micro satellite analysis. The nature of the hESC‐derived fibroblasts was identified by the expression of specific markers. This feeder system permits continuous growth of undifferentiated and pluripotent hESCs, as demonstrated by the expression of specific hESC markers, by the formation of teratomas after injection of hESCs into severely combined immunodeficient mice, and by in vitro differentiation of hESCs into differentiated cells of ectodermal, endodermal, and mesodermal origin. Feeder cells derived from hESCs offers a potentially more secure autogeneic and genotypically homogenous system for the growth of undifferentiated hESCs.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here