English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Cells derived from embryonic mouse STO cell lines differentiate into hepatocytes when transplanted into the livers of nonimmunosuppressed dipeptidylpeptidase IV (DPPIV)–negative F344 rats. Within 1 day after intrasplenic injection, donor cells moved rapidly into the liver and were found in intravascular and perivascular sites; by 1 month, they were intrasinusoidal and also integrated into hepatic plates with approximately 2% efficiency and formed conjoint bile canaliculi. Neither donor cell proliferation nor host inflammatory responses were observed during this time. Detection of intrahepatic mouse  COX1  mitochondrial DNA and mouse albumin mRNA in recipient rats indicated survival and differentiation of donor cells for at least 3 months. Mouse  COX1  targets were also detected intrahepatically 4–9 weeks after STO cell injection into nonimmunosuppressed wild‐type rats. In contrast to STO‐transplanted rats, mouse DNA or RNA was not detectable in untreated or mock‐transplanted rats or in rats injected with donor cell DNA. In cultured STO donor cells, DPPIV and glucose‐6‐phosphatase activities were observed in small clusters; in contrast, mouse major histocompatibility complex class I H‐2K q , H‐2D q , and H‐2L q  and class II I‐A q  markers were undetectable in vitro before or after interferon gamma treatment. Together with H‐2K allele typing, which confirmed the Swiss mouse origin of the donor cells, these observations indicate that mouse‐derived STO cell lines can differentiate along hepatocytic lineage and engraft into rat liver across major histocompatibility barriers.

Embryonic Mouse STO Cell–Derived Xenografts Express Hepatocytic Functions in the Livers of Nonimmunosuppressed Adult Rats