Open AccessAnalysis of Different Promoter Systems for Efficient Transgene Expression in Mouse Embryonic Stem Cell Lines
Author(s) -
Chung Sangmi,
Andersson Therese,
Sonntag KaiC.,
Björklund Lars,
Isacson Ole,
Kim KwangSoo
Publication year - 2002
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.20-2-139
Subject(s) - biology , embryonic stem cell , reporter gene , enhancer , promoter , transfection , transgene , microbiology and biotechnology , embryoid body , cellular differentiation , cell culture , gene expression , stem cell , gene , genetics , adult stem cell
Mouse embryonic stem (ES) cells are derived from the inner cell mass of the preimplantation embryo and have the developmental capacity to generate all cell types of the body. Combined with efficient genetic manipulation and in vitro differentiation procedures, ES cells are a useful system for the molecular analysis of developmental pathways. We analyzed and compared the transcriptional activities of a cellular polypeptide chain elongation factor 1 alpha (EF), a cellular‐virus hybrid (cytomegalo‐virus [CMV] immediate early enhancer fused to chicken β‐actin [CBA]), and a viral CMV promoter system in two ES cell lines. When transiently transfected, the EF and CBA promoters robustly drove reporter gene expression, while the CMV promoter was inactive. We also demonstrated that the EF and CBA promoters effectively drove gene expression in different stages of cell development: naïve ES cells, embryoid bodies (EBs), and neuronal precursor cells. In contrast, the CMV promoter did not have transcriptional activity in either ES cells or EB but had significant activity once ES cells differentiated into neuronal precursors. Our data show that individual promoters have different abilities to express reporter gene expression in the ES and other cell types tested.