Premium
KDM 6A and KDM 6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system
Author(s) -
Yang Lei,
Song Lishuang,
Liu Xuefei,
Bai Lige,
Li Guangpeng
Publication year - 2018
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201846240
Subject(s) - somatic cell nuclear transfer , reprogramming , biology , xist , demethylase , microbiology and biotechnology , cloning (programming) , gene knockdown , maternal to zygotic transition , embryonic stem cell , embryo , genetics , histone , blastocyst , cell culture , embryogenesis , zygote , cell , x inactivation , gene , x chromosome , programming language , computer science
Despite the success of animal cloning by somatic cell nuclear transfer ( SCNT ) in many species, the method is limited by its low efficiency. After zygotic genome activation ( ZGA ) during mouse development, a large number of endogenous retroviruses ( ERV s) are expressed, including the murine endogenous retrovirus‐L (Mu ERVL / MERVL ). In this study, we generate a series of MERVL reporter mouse strains to detect the ZGA event in embryos. We show that the majority of SCNT embryos do not undergo ZGA , and H3K27me3 prevents SCNT reprogramming. Overexpression of the H3K27me3‐specific demethylase KDM 6A, but not of KDM 6B, improves the efficiency of SCNT . Conversely, knockdown of KDM 6B not only facilitates ZGA , but also impedes ectopic Xist expression in SCNT reprogramming. Furthermore, knockdown of KDM 6B increases the rate of SCNT ‐derived embryonic stem cells from Duchenne muscular dystrophy embryos. These results not only provide insight into the mechanisms underlying failures of SCNT , but also may extend the applications of SCNT .