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Membrane fission by dynamin: what we know and what we need to know
Author(s) -
Antonny Bruno,
Burd Christopher,
De Camilli Pietro,
Chen Elizabeth,
Daumke Oliver,
Faelber Katja,
Ford Marijn,
Frolov Vadim A,
Frost Adam,
Hinshaw Jenny E,
Kirchhausen Tom,
Kozlov Michael M,
Lenz Martin,
Low Harry H,
McMahon Harvey,
Merrifield Christien,
Pollard Thomas D,
Robinson Phillip J,
Roux Aurélien,
Schmid Sandra
Publication year - 2016
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201694613
Subject(s) - dynamin , gtpase , fission , endocytosis , gtp' , microbiology and biotechnology , organelle , biology , biophysics , biochemistry , cell , physics , enzyme , nuclear physics , neutron
The large GTP ase dynamin is the first protein shown to catalyze membrane fission. Dynamin and its related proteins are essential to many cell functions, from endocytosis to organelle division and fusion, and it plays a critical role in many physiological functions such as synaptic transmission and muscle contraction. Research of the past three decades has focused on understanding how dynamin works. In this review, we present the basis for an emerging consensus on how dynamin functions. Three properties of dynamin are strongly supported by experimental data: first, dynamin oligomerizes into a helical polymer; second, dynamin oligomer constricts in the presence of GTP ; and third, dynamin catalyzes membrane fission upon GTP hydrolysis. We present the two current models for fission, essentially diverging in how GTP energy is spent. We further discuss how future research might solve the remaining open questions presently under discussion.