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The Arabidopsis CERK 1‐associated kinase PBL 27 connects chitin perception to MAPK activation
Author(s) -
Yamada Kenta,
Yamaguchi Koji,
Shirakawa Tomomi,
Nakagami Hirofumi,
Mine Akira,
Ishikawa Kazuya,
Fujiwara Masayuki,
Narusaka Mari,
Narusaka Yoshihiro,
Ichimura Kazuya,
Kobayashi Yuka,
Matsui Hidenori,
Nomura Yuko,
Nomoto Mika,
Tada Yasuomi,
Fukao Yoichiro,
Fukamizo Tamo,
Tsuda Kenichi,
Shirasu Ken,
Shibuya Naoto,
Kawasaki Tsutomu
Publication year - 2016
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201694248
Subject(s) - biology , mapk/erk pathway , microbiology and biotechnology , mapk cascade , kinase , chitin , protein kinase a , map kinase kinase kinase , phosphorylation , signal transduction , mitogen activated protein kinase kinase , mitogen activated protein kinase , biochemistry , chitosan
Perception of microbe‐associated molecular patterns by host cell surface pattern recognition receptors ( PRR s) triggers the intracellular activation of mitogen‐activated protein kinase ( MAPK ) cascades. However, it is not known how PRR s transmit immune signals to MAPK cascades in plants. Here, we identify a complete phospho‐signaling transduction pathway from PRR ‐mediated pathogen recognition to MAPK activation in plants. We found that the receptor‐like cytoplasmic kinase PBL 27 connects the chitin receptor complex CERK 1‐ LYK 5 and a MAPK cascade. PBL 27 interacts with both CERK 1 and the MAPK kinase kinase MAPKKK 5 at the plasma membrane. Knockout mutants of MAPKKK 5 compromise chitin‐induced MAPK activation and disease resistance to Alternaria brassicicola . PBL 27 phosphorylates MAPKKK 5 in vitro , which is enhanced by phosphorylation of PBL 27 by CERK 1. The chitin perception induces disassociation between PBL 27 and MAPKKK 5 in vivo . Furthermore, genetic evidence suggests that phosphorylation of MAPKKK 5 by PBL 27 is essential for chitin‐induced MAPK activation in plants. These data indicate that PBL 27 is the MAPKKK kinase that provides the missing link between the cell surface chitin receptor and the intracellular MAPK cascade in plants.