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Stabilization of the metaphase spindle by Cdc14 is required for recombinational DNA repair
Author(s) -
Villoria María Teresa,
Ramos Facundo,
Dueñas Encarnación,
Faull Peter,
Cutillas Pedro Rodríguez,
ClementeBlanco Andrés
Publication year - 2016
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201593540
Subject(s) - biology , metaphase , microbiology and biotechnology , dna , genetics , gene , chromosome
Cells are constantly threatened by multiple sources of genotoxic stress that cause DNA damage. To maintain genome integrity, cells have developed a coordinated signalling network called DNA damage response ( DDR ). While multiple kinases have been thoroughly studied during DDR activation, the role of protein dephosphorylation in the damage response remains elusive. Here, we show that the phosphatase Cdc14 is essential to fulfil recombinational DNA repair in budding yeast. After DNA double‐strand break ( DSB ) generation, Cdc14 is transiently released from the nucleolus and activated. In this state, Cdc14 targets the spindle pole body ( SPB ) component Spc110 to counterbalance its phosphorylation by cyclin‐dependent kinase (Cdk). Alterations in the Cdk/Cdc14‐dependent phosphorylation status of Spc110, or its inactivation during the induction of a DNA lesion, generate abnormal oscillatory SPB movements that disrupt DSB ‐ SPB interactions. Remarkably, these defects impair DNA repair by homologous recombination indicating that SPB integrity is essential during the repair process. Together, these results show that Cdc14 promotes spindle stability and DSB ‐ SPB tethering during DNA repair, and imply that metaphase spindle maintenance is a critical feature of the repair process.