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Aim:  The development of calixarene based phenol biosensor.    Methods:  This study describes the application of a calixarene derivative, 5,17-diamino-25,27-bis(3-thiol-1-oxypropane)-26,28-dihydroxycalix[4]arene (HS-Calix-NH    Results:  The linearity was observed in the range of 0.1–100 μM and 1.0–100 μM for catechol and phenol, respectively. The potential use of the biosensor was investigated for phenol analysis in artificial samples which simulate the industrial waste water, which is highly acidic and composed of concentrated salt, without needing any sample pre-treatment step.    Conclusion:  The prepared Lac biosensor has a potential for rapid, selective and easy detection of phenolic contaminations in samples.

A novel immobilization matrix for the biosensing of phenol: self assembled monolayers of calixarenes