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Toxoplasmosis is a major disease caused by    Methods:  PAA and SAG1 were covalently conjugated using a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines. To demonstrate conjugation of these molecules in size exclusion chromatography (SEC), fluorescence spectroscopy was used.    Results:  For the first time, one of the most important surface antigens of    Discussion and conclusion:  The obtained SAG1-PAA conjugate can be used for vaccine development against

turkish journal of biochemistry

Development and characterization of a formulation based covalent conjugation with polyacrylic acid and recombinant major surface antigen (SAG1) of  Toxoplasma gondii