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Rapid climate change is currently altering species distribution ranges. Evaluating the long-term stress level in wild species undergoing range expansion may help better understanding how species cope with the changing environment. Here, we focused on the white-footed mouse ( Peromyscus leucopus ), a widespread small mammal species in North-America whose distribution range is rapidly shifting northward. We evaluated long-term stress level in several populations of  P. leucopus  in Quebec (Canada), from the northern edge of the species distribution to more core populations in Southern Quebec. We first tested the hypothesis that populations at the range margin are under higher stress than more established populations in the southern region of our study area. We then compared four measures of long-term stress level to evaluate the congruence between these commonly used methods. We did not detect any significant geographical trend in stress level across our study populations of  P. leucopus . Most notably, we found no clear congruence between the four measures of stress level we used, and conclude that these four commonly used methods are not equivalent, thereby not comparable across studies.

Long-term stress level in a small mammal species undergoing range expansion