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This report describes the high yield biotransformation of 1,8-cineole by the strain Gymnopilus spectabilis 7423, a common fungus isolated from the Eucalyptus tree. The biotransformation was conducted under resting cell conditions and different parameters were tested in order to achieve up to 90% bioconversion. Only two regioisomers were detected, and they were identified as 3-α-hydroxy-1,8-cineole and 2-α-hydroxy-1,8- cineole obtained in a 82:8 ratio.

3-Hydroxycineole bioproduction from 1,8-cineole using Gymnopilus spectabilis 7423 under resting cell conditions