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Light is arguably one of the most significant aspects of microgreen algae growth and lipid yields. Microgreen algae-based biodiesel production offers a pioneering method of producing renewable fuel. In this research,  Chlorella  sp. was cultivated in colorless (control), red, orange, yellow, blue, and green Erlenmeyer flasks. The BG11 medium was used with continuous illumination by cool white fluorescent light with a light intensity of 2,500 lux (35 µmol photon·m −2 ·s −1 ), and light: dark (12:12 h). After 14 days of cultivation, dry weight, pigments, and lipids % were measured. The results demonstrate that the red color was the best effect on  Chlorella  sp. growth under continuous light. Control enhanced the production of Chlorophyll- a  and Chlorophyll- b  in continuous illumination. Non-significant results among control, red, and blue colors in the case of algal dry weight, under dark: light. The quantity of saturated fatty acids was higher in algae cultivated in the red flask (42.9%), followed by blue with continuous illumination. Control with continuous illumination denotes a higher amount of monounsaturated fatty acids. Alga in the blue flask produced a high quantity (83.71%) of polyunsaturated fatty acids in the light: dark cycle. The color light can be used to enhance the lipid quality.

Influence of different colors with light regime on Chlorella sp., biomass, pigments, and lipids quantity and quality