Open AccessA Patient With Sporotrichosis Diagnosed By Molecular Biology Combined With Traditional Methods
Author(s) -
Runchao Wang,
Haixia Jing,
Chen Shaoxiu,
Yao Fang Yang,
Hualiang Nan,
Tingting Chen
Publication year - 2019
Publication title -
journal of medical cases
Language(s) - English
Resource type - Journals
eISSN - 1923-4163
pISSN - 1923-4155
DOI - 10.14740/jmc3362
Subject(s) - sporotrichosis , sporothrix schenckii , genbank , medicine , pathology , dna extraction , fungal pathogen , dermatology , biology , microbiology and biotechnology , polymerase chain reaction , pathogen , immunology , genetics , gene
We present a case of sporotrichosis diagnosed by molecular biology combined with traditional methods. Fungal DNA was extracted from skin tissues of the patient by fungal DNA extraction kit. The PCR amplified fragments were compared on GenBank website, and the pathogenic fungi were identified. This case was diagnosed by molecular biology, clinical manifestations, fungal morphology and pathology. The pathogenic fungi of the patient was identified as Sporothrix globosa by comparison, combined with typical clinical manifestations, typical filamentous colonies by fungi culture and pathological findings of infectious granuloma. The case could be diagnosed as Sporothrix globosa -induced sporotrichosis. Molecular biology combined with traditional detection methods for diagnosis of sporotrichosis is helpful to improve the accuracy of diagnosis.