Open AccessThe role of XTRAP-gamma in Xenopus pronephros development
Author(s) -
Donghui Li,
Techuan Chan,
Reiko Satow,
Shinji Komazaki,
Kouhei Hashizume,
Makoto Asashima
Publication year - 2005
Publication title -
the international journal of developmental biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.837
H-Index - 103
eISSN - 1696-3547
pISSN - 0214-6282
DOI - 10.1387/ijdb.052005dl
Subject(s) - pronephros , biology , morpholino , xenopus , mesonephros , gene knockdown , microbiology and biotechnology , intermediate mesoderm , kidney development , retinoic acid , medicine , endocrinology , gastrulation , gene , embryogenesis , genetics , embryo , embryonic stem cell
We isolated and characterized the Xenopus translocon-associated protein XTRAP-gamma, one of four subunits of the translocon-associated protein complex. TRAP has been proposed to aid the translocation of nascent polypeptides into the lumen of the endoplasmic reticulum, but this has not been demonstrated until now. XTRAP-gamma was specifically expressed in the pronephros tubules of Xenopus laevis from stage 25 during kidney development. Antisense morpholino oligonucleotide-mediated knockdown of XTRAP-gamma suppressed tubulogenesis and decreased expression of the pronephros marker genes Pax-2 and Wnt-4. XTRAP-gamma morpholinos also inhibited differentiation of the pronephros in activin/retinoic acid-treated animal caps. We conclude that XTRAP-gamma plays an important role in the process of pronephros differentiation.