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The low diversity of the D-subgenome of bread wheat requires the involvement of new alleles for breeding. In grasses, the allelic state of  Growth Regulating Factor  ( GRF ) gene is correlated with nitrogen uptake. In this study, we characterized the sequence of  TaGRF-2D  and assessed its diversity in bread wheat and goatgrass  Aegilops tauschii  (genome DD).  In silico  analysis was performed for reference sequence searching, primer pairs design and sequence assembly. The gene sequence was obtained using Illumina and Sanger sequencing. The complete sequences of  TaGRF-2D  were obtained for 18 varieties of wheat. The polymorphism in the presence/absence of two GCAGCC repeats in 5’ UTR was revealed and the GRF-2D-SSR marker was developed. Our results showed that the alleles 5’ UTR-250 and 5’ UTR-238 were present in wheat varieties, 5’ UTR-250 was presented in the majority of wheat varieties. In  Ae .  tauschii  ssp.  strangulata  (likely donor of the D-subgenome of polyploid wheat), most accessions carried the 5’ UTR-250 allele, whilst most  Ae .  tauschii  ssp.  tauschii  have 5’ UTR-244. The developed GRF-2D-SSR marker can be used to study the genetic diversity of wheat and  Ae .  tauschii .

Allele mining of TaGRF-2D gene 5’-UTR in Triticum aestivum and Aegilops tauschii genotypes