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Phytophthora capsici  is a soil borne pathogen, and is among the most destructive pathogens for  Capsicum annuum  (chile).  P .  capsici  is known to cause diseases on all parts of the chile plants. Therefore, it requires independent resistance genes to control disease symptoms that are induced by each of the  P .  capsici  strains. This requirement of multiple resistance genes to confer resistance to  P .  capsici , in chile makes breeding for resistance a daunting pursuit. Against this backdrop, a genetic engineering approach would be to introduce a broad host resistance gene into chile in order to protect it from different races of  P .  capsici . Notably, a broad host resistance gene  RB  from  Solanum bulbocastanum  has been shown to confer resistance to  P .  infestans  in both  S .  tuberosum  and  S .  lycopersicum . We agroinfiltrated the  RB  gene into the leaves of susceptible chile plants, demonstrating that the gene is also capable of lending resistance to  P .  capsici  in chile. We introduced the  RB  gene into chile by developing an  Agrobacterium tumefaciens  mediated transformation system. The integration of the  RB  gene into the genome of the primary transformants and its subsequent transfer to the F1 generation was confirmed by genomic PCR using primers specific for the  RB  gene. A 3:1 ratio for the presence and absence of the  RB  gene was observed in the F1 progeny. In addition to showing resistance to  P .  capsici  in a leaf inoculation experiment, about 30% of the F1 progeny also exhibited resistance to root inoculation. Our data, when taken together, suggests that the  RB  gene from  S .  bulbocastanum  confers resistance against  P .  capsici  in  C .  annuum , thereby demonstrating that the  RB  gene has an even broader host range than reported in the literature–both in terms of the host and the pathogen.

Chile (Capsicum annuum) plants transformed with the RB gene from Solanum bulbocastanum are resistant to Phytophthora capsici