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The capture of antimicrobial resistance genes (ARGs) by mobile genetic elements (MGEs) plays a critical role in resistance acquisition for human-associated bacteria. Although aquaculture environments are recognized as important reservoirs of ARGs, intra- and intercellular mobility of MGEs discovered in marine organisms is poorly characterized. Here, we show a new pattern of interspecies ARGs transfer involving a ‘non-conjugative’ integrative element. To identify active MGEs in a  Vibrio ponticus  isolate, we conducted whole-genome sequencing of a transconjugant obtained by mating between  Escherichia coli  and  Vibrio ponticus . This revealed integration of a plasmid (designated pSEA1) into the chromosome, consisting of a self-transmissible plasmid backbone of the MOB H  group, ARGs, and a 13.8-kb integrative element Tn 6283 . Molecular genetics analysis suggested a two-step gene transfer model. First, Tn 6283  integrates into the recipient chromosome during suicidal plasmid transfer, followed by homologous recombination between the Tn 6283  copy in the chromosome and that in the newly transferred pSEA1. Tn 6283  is unusual among integrative elements in that it apparently does not encode transfer function and its excision barely generates unoccupied donor sites. Thus, its movement is analogous to the transposition of insertion sequences rather than to that of canonical integrative and conjugative elements. Overall, this study reveals the presence of a previously unrecognized type of MGE in a marine organism, highlighting diversity in the mode of interspecies gene transfer.

plos one

Interplay of a non-conjugative integrative element and a conjugative plasmid in the spread of antibiotic resistance via suicidal plasmid transfer from an aquaculture Vibrio isolate