Open AccessSurface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes
Author(s) -
Giancarlo Margheri,
Riccardo d’Agostino,
Lucia Becucci,
Rolando Guidelli,
Bruno Tiribilli,
Mario Del Rosso
Publication year - 2012
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.3.003119
Subject(s) - lipid raft , surface plasmon resonance , cholera toxin , lipid bilayer , ganglioside , membrane , biophysics , chemistry , materials science , nanotechnology , biology , biochemistry , nanoparticle , microbiology and biotechnology
A procedure based on surface plasmon resonance (SPR) is proposed to monitor the lateral mobility of lipid molecules in solid-supported bilayer lipid membranes (ssBLMs), an essential prerequisite for the formation of important microdomains called lipid rafts (LRs). The procedure relies on the marked tendency of the ganglioside GM1 to be recruited by LRs and to act as a specific receptor of the beta-subunit of the cholera toxin (ChTB). In the presence of both GM1 and ChTB, spontaneous formation of lipid rafts domains in mobile ssBLMs is accompanied by an appreciable increase in the amount of adsorbed ChTB, as monitored by SPR.