Open AccessCell death detection by quantitative three-dimensional single-cell tomography
Author(s) -
Nai-Chia Cheng,
TsungHsun Hsieh,
Yu-Ta Wang,
ChienChih Lai,
Chun-Lin Chang,
Ming-Yi Lin,
DingWei Huang,
Jeng-Wei Tjiu,
Sheng-Lung Huang
Publication year - 2012
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.3.002111
Subject(s) - optical coherence tomography , single cell analysis , tomography , in situ , optics , cell , chemistry , physics , biochemistry , organic chemistry
Ultrahigh-resolution optical coherence tomography (UR-OCT) has been used for the first time to our knowledge to study single-cell basal cell carcinoma (BCC) in vitro. This noninvasive, in situ, label-free technique with deep imaging depth enables three-dimensional analysis of scattering properties of single cells with cellular spatial resolution. From three-dimensional UR-OCT imaging, live and dead BCC cells can be easily identified based on morphological observation. We developed a novel method to automatically extract characteristic parameters of a single cell from data volume, and quantitative comparison and parametric analysis were performed. The results demonstrate the capability of UR-OCT to detect cell death at the cellular level.