Open AccessMeasurement of orientation and susceptibility ratios using a polarization-resolved second-harmonic generation holographic microscope
Author(s) -
David G. Winters,
David R. Smith,
Philip Schlup,
Randy A. Bartels
Publication year - 2012
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.3.002004
Subject(s) - holography , polarization (electrochemistry) , optics , second harmonic generation , microscopy , materials science , spectrum analyzer , microscope , excitation , second harmonic imaging microscopy , spatial frequency , physics , chemistry , laser , quantum mechanics
Three-dimensional second-harmonic fields, sample orientation, and susceptibility ratios of biological samples are measured using polarization-resolved second-harmonic generation (SHG) microscopy. The three-dimensional (3D) polarization is gathered by measurement of a series of holograms for which excitation and analyzer polarizations are systematically varied, and the 3D SHG field is recovered through numerical back propagation. Harmonophore orientation is resolved in 3D from a sub-set of polarization-resolved SHG holograms. We further expand on previous approaches for the determination of susceptibility ratios, adding the calculation of multiple ratio values to allow intrinsic verification.