Open AccessCross-phase modulation spectral shifting: nonlinear phase contrast in a pump-probe microscope
Author(s) -
Jesse W. Wilson,
Prathyush Samineni,
Warren S. Warren,
Martin C. Fischer
Publication year - 2012
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.3.000854
Subject(s) - optics , microscope , phase modulation , materials science , phase (matter) , self phase modulation , modulation (music) , microscopy , cross phase modulation , nonlinear system , phase contrast microscopy , contrast (vision) , context (archaeology) , optical microscope , phase contrast imaging , nonlinear optics , physics , laser , phase noise , acoustics , scanning electron microscope , paleontology , quantum mechanics , biology
Microscopy with nonlinear phase contrast is achieved by a simple modification to a nonlinear pump-probe microscope. The technique measures cross-phase modulation by detecting a pump-induced spectral shift in the probe pulse. Images with nonlinear phase contrast are acquired both in transparent and absorptive media. In paraffin-embedded biopsy sections, cross-phase modulation complements the chemically-specific pump-probe images with structural context.