Open AccessMulticolour single molecule imaging on cells using a supercontinuum source
Author(s) -
S Webb,
Laura C. Zanetti-Domingues,
Benjamin C. Coles,
Daniel J. Rolfe,
Richard J. Wareham,
Marisa L. Martin-Fernandez
Publication year - 2012
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.3.000400
Subject(s) - supercontinuum , fluorescence , laser , optics , multiplexing , materials science , wavelength , fluorescence lifetime imaging microscopy , optoelectronics , physics , computer science , telecommunications , photonic crystal fiber
Multicolour single molecule fluorescence imaging enables the study of multiple proteins in the membranes of living cells. We describe the use of a supercontinuum laser as the excitation source, show its comparability with multiplexed single-wavelength lasers and demonstrate that it can be used to study membrane proteins such as the ErbB receptor family. We discuss the benefits of white-light sources for single molecule fluorescence, in particular their ease of use and the freedom to use the most appropriate dye without being constrained by available laser wavelengths.