Open AccessIn vivo volumetric imaging of chicken retina with ultrahigh-resolution spectral domain optical coherence tomography
Author(s) -
Alireza Akhlagh Moayed,
Sanaz Hariri,
Eun Song Song,
Vivian Choh,
Kostadinka Bizheva
Publication year - 2011
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.2.001268
Subject(s) - retina , optical coherence tomography , retinal , preclinical imaging , image resolution , ganglion cell layer , tomography , optics , materials science , biomedical engineering , in vivo , ophthalmology , medicine , biology , physics , microbiology and biotechnology
The chicken retina is an established animal model for myopia and light-associated growth studies. It has a unique morphology: it is afoveate and avascular; oxygen and nutrition to the inner retina is delivered by a vascular tissue (pecten) that protrudes into the vitreous. Here we present, to the best of our knowledge, the first in vivo, volumetric high-resolution images of the chicken retina. Images were acquired with an ultrahigh-resolution optical coherence tomography (UHROCT) system with 3.5 µm axial resolution in the retina, at the rate of 47,000 A-scans/s. Spatial variations in the thickness of the nerve fiber and ganglion cell layers were mapped by segmenting and measuring the layer thickness with a semi-automatic segmentation algorithm. Volumetric visualization of the morphology and morphometric analysis of the chicken retina could aid significantly studies with chicken retinal models of ophthalmic diseases.