Open AccessIntravital Two-photon Imaging of Dynamic Alteration of Hepatic Lipid Droplets in Fasted and Refed State
Author(s) -
Jaekyun Moon,
Pilhan Kim
Publication year - 2021
Publication title -
journal of lipid and atherosclerosis
Language(s) - English
Resource type - Journals
eISSN - 2288-2561
pISSN - 2287-2892
DOI - 10.12997/jla.2021.10.3.313
Subject(s) - in vivo , intravital microscopy , lipid metabolism , lipid droplet , medicine , pathology , chemistry , endocrinology , biology , microbiology and biotechnology
The liver plays a central role in lipid metabolism. During fasting and feeding, the fatty acid trafficking between adipose tissue and liver induces accumulation and dissociation of dynamic hepatic lipid droplets (LDs). Herein, we established an intravital 2-photon imaging technique to longitudinally visualize the dynamic in vivo alteration of hepatic LD deposition during fasting and refeeding in the liver of live mouse.