Open AccessDevelopment and study of perspective clinical properties of biologically active matrix composed of decellularized lyophilized human amniotic membrane in combination with platelet-derived growth factors and hyaluronic acid
Author(s) -
H. O. Vyshnevska,
Zurab Kakabadze,
S. A. Schnayder,
О. І. Тірон,
O. F. Dzygal
Publication year - 2019
Publication title -
journal of education, health and sport
Language(s) - English
Resource type - Journals
ISSN - 2391-8306
DOI - 10.12775/jehs.2019.09.11.038
Subject(s) - decellularization , hyaluronic acid , chemistry , membrane , regeneration (biology) , sodium dodecyl sulfate , amnion , extracellular matrix , regenerative medicine , microbiology and biotechnology , tissue engineering , matrix (chemical analysis) , biomedical engineering , biochemistry , biophysics , cell , chromatography , anatomy , biology , medicine , fetus , pregnancy , genetics
Nowadays the development of novel biomaterials which would promote tissue lesions regeneration is one of the crucial tasks of regenerative medicine. In the current research we developed a biological active membrane composed of decellularized, lyophilized human amniotic membrane in combination with growth factors of platelet-rich plasma (PRP) and hyaluronic acid. On the basis of histological examination, scanning electron microscopic examination and DNA content analyses it was drawn a conclusion that Sodium Dodecyl Sulfate (SDS) (Sigma) solution and 1% Triton X-100 solution completely remove all existing cells of placenta and chorionic-amniotic membrane without impairment of their structure. After the processes of decellularization and lyophilization the DNA content was less than 2%. The PRP growth factors as the components biological matrix provide faster regeneration. The latter are fixed on the surface of cellular structure of decellularized, lyophilized human amniotic membrane by the reservoir of gelatinous hyaluronic acid