English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background Listeria monocytogenes  is a foodborne pathogen that causes listeriosis in humans. This pathogen activates multiple regulatory mechanisms in response to stress, and cobalamin biosynthesis might have a potential role in bacterial protection. Low temperature is a strategy used in the food industry to control bacteria proliferation; however,  L. monocytogenes  can grow in cold temperatures and overcome different stress conditions. In this study we selected  L. monocytogenes  List2-2, a strain with high tolerance to the combination of low temperature + copper, to understand whether the cobalamin biosynthesis pathway is part of the tolerance mechanism to this stress condition. For this, we characterized the transcription level of three cobalamin biosynthesis-related genes ( cbiP ,  cbiB,  and  cysG ) and the  eutV  gene, a transcriptional regulator encoding gene involved in ethanolamine metabolism, in  L. monocytogenes  strain List2-2 growing simultaneously under two environmental stressors: low temperature (8 °C) + copper (0.5 mM of CuSO 4  × 5H 2 O). In addition, the gene  cbiP , which encodes an essential cobyric acid synthase required in the cobalamin pathway, was deleted by homologous recombination to evaluate the impact of this gene in  L. monocytogenes  tolerance to a low temperature (8 °C) + different copper concentrations. Results By analyzing the KEGG pathway database, twenty-two genes were involved in the cobalamin biosynthesis pathway in  L. monocytogenes  List2-2. The expression of genes  cbiP ,  cbiB,  and  cysG,  and  eutV  increased 6 h after the exposure to low temperature + copper. The cobalamin  cbiP  mutant strain List2-2Δ cbiP  showed less tolerance to low temperature + copper (3 mM) than the wild-type  L. monocytogenes  List2-2. The addition of cyanocobalamin (5 nM) to the medium reverted the phenotype observed in List2-2Δ cbiP . Conclusion These results indicate that cobalamin biosynthesis is necessary for  L. monocytogenes  growth under stress and that the  cbiP  gene may play a role in the survival and growth of  L. monocytogenes  List2-2 at low temperature + copper.

Cobalamin cbiP mutant shows decreased tolerance to low temperature and copper stress in Listeria monocytogenes