English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Actin filament-associated protein 1 antisense RNA 1 ( AFAP1-AS1 ), a long noncoding RNA, is significantly highly expressed and associated with metastasis and poor prognosis in many cancers, including nasopharyngeal carcinoma (NPC). In this study, we aim to identify the role of  AFAP1-AS1  acting as an oncogenic lncRNA to promote NPC metastasis.    Methods  The role of  AFAP1-AS1 , miR-423-5p, and   FOSL2  in NPC metastasis was investigated in vitro and in vivo. Bioinformatics analysis and luciferase activity assays were used to identify the interaction between  AFAP1-AS1 , miR-423-5p, and  FOSL2 . Additionally, real-time PCR and western blotting were used to assess the function of  AFAP1-AS1  acting as an oncogenic lncRNA to promote NPC progression by regulating miR-423-5p and the downstream Rho/Rac pathway.    Results  In this study, we determined that  AFAP1-AS1  functions as a competing endogenous RNA in NPC to regulate the Rho/Rac pathway through miR-423-5p. These interactions can mediate the expression of  RAB11B ,  LASP1 , and  FOSL2  and accelerate cell migration and invasion via the Rho/Rac signaling pathway or  FOSL2 .  AFAP1-AS1  and  FOSL2  could competitively bind with miR-423-5p to regulate several molecules, including  RAB11B  and  LASP1  of the Rho/Rac signaling pathway.  AFAP1-AS1  can also regulate the expression of  LASP1,  which was transcriptionally regulated by  FOSL2 , resulting in increased migration and invasion of NPC cells via the Rho/Rac signaling pathway.    Conclusions  The observations in this study identify an important role for  AFAP1-AS1  as a competing endogenous RNA (ceRNA) in NPC pathogenesis and indicate that it may serve as a potential target for cancer diagnosis and treatment.    Electronic supplementary material  The online version of this article (10.1186/s13046-018-0918-9) contains supplementary material, which is available to authorized users.

journal of experimental and clinical cancer research

Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway