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Background   Ganoderma lucidum , a well-known medicinal mushroom, has received wide attention as a promising cell factory for producing bioactive compounds. However, efficient expression of heterologous genes remains a major challenge in  Ganoderma , hindering metabolic regulation research and molecular breeding of this species.    Results  We show that the presence of glyceraldehyde-3-phosphate dehydrogenase gene ( gpd ) intron 1 at the 5′ end of, the 3′ end of, or within the heterologous phosphinothricin-resistant gene ( bar ) is efficient for its expression in  G. lucidum . The enhanced expression of  bar  is exhibited by the higher accumulation of mRNA and increased amounts of protein. Moreover, the insertion of the  gpd  intron 1 in the β-glucuronidase gene ( gus ) elevates its mRNA accumulation and enzyme activity, which facilitates the use of this reporter gene in  Ganoderma .    Conclusions  This study has demonstrated the importance of the introduction of  gpd  intron 1 for the efficient expression of  bar  and  gus  in  G. lucidum . The presence of the  gpd  intron 1 in heterologous genes increases levels of mRNA accumulation and protein expression in basidiomycete  Ganoderma . The developed method may be utilized in upregulating the expression of other heterologous genes in  Ganoderma .    Supplementary Information  The online version contains supplementary material available at 10.1186/s12934-021-01654-8.

microbial cell factories

Efficient expression of heterologous genes by the introduction of the endogenous glyceraldehyde-3-phosphate dehydrogenase gene intron 1 in Ganoderma lucidum