English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background Cryptosporidium  spp. are obligate intracellular apicomplexan parasites transmitted to humans and other animals by contaminated water, food, or direct contact. They mainly cause gastrointestinal symptoms, although subclinical infections are also common. Cats are primarily infected by host-adapted  Cryptosporidium felis  while  C. parvum  and  C. muris  have also been detected in some cases. In this study, the molecular prevalence of  Cryptosporidium  spp. was investigated by screening 399 fecal samples collected from stray cats using nested PCR targeting the 18S rRNA gene for the first time in Turkey. Additionally,  Cryptosporidium  PCR-positive samples were genotyped by nested PCR- restriction fragment length polymorphism (RFLP), and subsequently, amplicons of 18S SSU rRNA were sequenced. They were further subtyped by amplification and sequencing of the  gp60  gene. Results Among fecal samples screened, 12 of them (3%) were found to be  Cryptosporidium-positive , and according to RFLP and sequencing of  18S rRNA  gene, all positive samples were identified as  C. felis . Subtyping analyses at the  gp60  gene showed that  C. felis  isolates belonged to the XIXa subtype family, which are closely related to human subtypes of the parasite. Conclusions The results of this study are important in terms of indicating the potential role of stray cats for transmission of  Cryptosporidium  spp. to humans or other animals. Also, the presence of XIXa, which is the dominant subtype family of  C. felis  in cats and humans was shown for the first time in stray cats of İzmir, Turkey.

Molecular prevalence and subtyping of Cryptosporidium spp. in fecal samples collected from stray cats in İzmir, Turkey