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Background Pathogenic  Escherichia coli  ( E. coli ) is an important causative agent for infectious diseases in pigs and causes significant economic loss. The global concern of antimicrobial resistance of bacteria raises awareness of the alternative ways of using antimicrobial peptides (AMPs). The study was aimed to identify and test the efficacy of AMPs from  Lactobacillus  spp. against the growth of pathogenic  E. coli  isolated from pigs in Thailand. Briefly, cell-free culture supernatants (CFCS) from 3 strains of lactic acid bacteria (LAB) consisting of  Lactobacillus acidophilus  (strain KMP),  Lactobacillus plantarum  (strain KMP), and  Pediococcus pentosaceus  (strain KMP) were tested against pathogenic  E. coli  via agar well diffusion assay in quadruplicates. The presence of a zone of inhibition (ZOI) around wells was evaluated at different incubation time. Acid and bile tolerance test was performed for bacterial viability in acid and bile salt conditions. In addition, LAB cross-streaking assay was evaluated for antagonist activity. Results The study showed that CFCS from  L. acidophilus  KMP,  L. plantarum  KMP, and  P. pentosaceus  KMP could inhibit the growth of pathogenic  E. coli  isolated from pigs in a time-dependent manner. To exemplify, the ZOI of  L. plantarum  KMP against  E. coli  (ETEC) at 8, 10, 12, 14, and 16 h incubation, were 26.6 ± 1.1, 24.9 ± 1.9, 22.5 ± 2.4, 20.3 ± 2.9, and 17.9 ± 3.3 mm, respectively. The ZOI was significantly different between 8, 10, 12, 14 h incubation, and the ZOI of the CFCS from  L. plantarum  KMP was larger than others ( P -value &lt; 0.05). Furthermore,  L. acidophilus  KMP,  L. plantarum  KMP, and  P. pentosaceus  KMP showed viability in pH 3.0, 0.3, and 0.5% (w/v) bile salt concentration. They exhibited no antagonist activity among each other. Conclusions According to the results, the CFCS from LAB including  L. acidophilus  KMP,  L. plantarum  KMP and  P. pentosaceus  KMP can inhibit the growth of pathogenic  E. coli , isolated from pigs in Thailand. The antimicrobial activity observed was incubation time dependent.

Cell-free culture supernatants of Lactobacillus spp. and Pediococcus spp. inhibit growth of pathogenic Escherichia coli isolated from pigs in Thailand