English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The unicellular red alga  Cyanidioschyzon merolae  exhibits a very simple cellular and genomic architecture. In addition, procedures for genetic modifications, such as gene targeting by homologous recombination and inducible/repressible gene expression, have been developed. However, only two markers for selecting transformants, uracil synthase ( URA ) and chloramphenicol acetyltransferase ( CAT ), are available in this alga. Therefore, manipulation of two or more different chromosomal loci in the same strain in  C. merolae  is limited.    Results  This study developed a nuclear targeting and transformant selection system using an antibiotics blasticidin S (BS) and the BS deaminase ( BSD ) selectable marker by homologous recombination in  C. merolae . In addition, this study has succeeded in simultaneously modifying two different chromosomal loci by a single-step cotransformation based on the combination of  BSD  and  CAT  selectable markers. A  C. merolae  strain that expresses mitochondrion-targeted  mSCARLET  (with the  BSD  marker) and  mVENUS  (with the  CAT  marker) from different chromosomal loci was generated with this procedure.    Conclusions  The newly developed  BSD  selectable marker enables an additional genetic modification to the already generated  C. merolae  transformants based on the  URA  or  CAT  system. Furthermore, the cotransformation system facilitates multiple genetic modifications .  These methods and the simple nature of the  C. merolae  cellular and genomic architecture will facilitate studies on several phenomena common to photosynthetic eukaryotes.    Supplementary Information  The online version contains supplementary material available at 10.1186/s12870-021-03365-z.

bmc plant biology

A cotransformation system of the unicellular red alga Cyanidioschyzon merolae with blasticidin S deaminase and chloramphenicol acetyltransferase selectable markers