English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background Citrus is one of the most important fresh fruit crops worldwide. Juice sac granulation is a physiological disorder, which leads to a reduction in soluble solid concentration, total sugar, and titratable acidity of citrus fruits. Pectin methylesterase (PME) catalyzes the de-methylesterification of homogalacturonans and plays crucial roles in cell wall modification during plant development and fruit ripening. Although  PME  family has been well investigated in various model plants, little is known regarding the evolutionary property and biological function of  PME  family genes in citrus. Results In this study, 53 non-redundant  PME  genes were identified from  Citrus sinensis  genome, and these  PME  genes were divided into four clades based on the phylogenetic relationship. Subsequently, bioinformatics analyses of gene structure, conserved domain, chromosome localization, gene duplication, and collinearity were performed on  CsPME  genes, providing important clues for further research on the functions of  CsPME  genes. The expression profiles of  CsPME  genes in response to juice sac granulation and low-temperature stress revealed that  CsPME g enes were involved in the low temperature-induced juice sac granulation in navel orange fruits. Subcellular localization analysis suggested that  CsPME  genes were localized on the apoplast, endoplasmic reticulum, plasma membrane, and vacuole membrane. Moreover, yeast one-hybrid screening and dual luciferase activity assay revealed that the transcription factor  CsRVE1  directly bound to the promoter of  CsPME3  and activated its activity. Conclusion In summary, this study conducts a comprehensive analysis of the  PME  gene family in citrus, and provides a novel insight into the biological functions and regulation patterns of  CsPME  genes during juice sac granulation of citrus.

Characterization of pectin methylesterase gene family and its possible role in juice sac granulation in navel orange (Citrus sinensis Osbeck)