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It is highly desirable to develop a rapid and simple method to detect pathogens. Combining nanomaterials with electrochemical techniques is an efficient way for pathogen detection. Herein, a novel 3D Ag nanoflower was prepared via a biomineralization method by using bovine serum albumin (BSA) as a template. It was adopted as a sensing interface to construct an electrochemical bacteria immunosensor for the rapid detection of foodborne pathogens  Escherichia coli  ( E. coli ) O157:H7. Bacterial antibody was immobilized onto the surface of Ag nanoflowers through covalent conjugation. Electrochemical impedance spectroscopy (EIS) was used to detect and validate the resistance changes, where [Fe(CN) 6 ] 3−/4−  acted as the redox probe. A linear relation between  R   et  and  E. coli  concentration was obtained in the  E. coli  concentration range of 3.0 × 10 2 –3.0 × 10 8  cfu mL −1 . The as-prepared biosensor gave rise to an obvious response to  E. coli  but had no distinct response to  Cronobacter sakazakii , methicillin-resistant  Staphylococcus aureus  (MRSA),  Staphylococcus albus ,  Lactobacillus easei , and  Shigella flexneri , revealing a high selectivity for the detection of the pathogens down to 100 cfu mL −1  in a short time. We believe that this BSA-conjugated 3D Ag nanoflowers could be used as a powerful interface material with good conductivity and biocompatibility for improving pathogen detection and treatment in the field of medicine, environment, and food safety.   Electronic supplementary material  The online version of this article (doi:10.1186/s11671-016-1711-3) contains supplementary material, which is available to authorized users.

Label-Free 3D Ag Nanoflower-Based Electrochemical Immunosensor for the Detection of Escherichia coli O157:H7 Pathogens