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Background  Probiotic bacteria are thought to play an important role in the digestive system and therefore have to survive the passage from stomach to intestines. Recently, a novel approach to simulate the passage from stomach to intestines in a single bioreactor was developed. The advantage of this automated one reactor system was the ability to test the influence of acid, bile salts and pancreatin.   Lactobacillus gasseri  K7 is a strain isolated from infant faeces with properties making the strain interesting for cheese production. In this study, a single reactor system was used to evaluate the survival of  L. gasseri  K7 and selected bifidobacteria from our collection through the stomach-intestine passage.    Results  Initial screening for acid resistance in acidified culture media showed a low tolerance of  Bifidobacterium dentium  for this condition indicating low survival in the passage. Similar results were achieved with  B. longum  subsp.  infantis  whereas  B. animalis  subsp.  lactis  had a high survival.  These initial results were confirmed in the bioreactor model of the stomach-intestine passage.  B. animalis  subsp.  lactis  had the highest survival rate (10%) attaining approximately 5 × 10 6  cfu ml -1  compared to the other tested bifidobacteria strains which were reduced by a factor of up to 10 6 .  Lactobacillus gasseri  K7 was less resistant than  B. animalis  subsp.  lactis  but survived at cell concentrations approximately 1000 times higher than other bifidobacteria.    Conclusion  In this study, we were able to show that  L. gasseri  K7 had a high survival rate in the stomach-intestine passage. By comparing the results with a previous study in piglets we could confirm the reliability of our simulation. Of the tested bifidobacteria strains, only  B. animalis  subsp.  lactis  showed acceptable survival for a successful passage in the simulation system.

Evaluation of the passage of Lactobacillus gasseri K7 and bifidobacteria from the stomach to intestines using a single reactor model