English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  DNA methylation is an important biological form of epigenetic modification, playing key roles in plant development and environmental responses.    Results  In this study, we examined single-base resolution methylomes of  Populus  under control and drought stress conditions using high-throughput bisulfite sequencing for the first time. Our data showed methylation levels of methylated cytosines, upstream 2kp, downstream 2kb, and repeatitive sequences significantly increased after drought treatment in  Populus . Interestingly, methylation in 100 bp upstream of the transcriptional start site (TSS) repressed gene expression, while methylations in 100-2000bp upstream of TSS and within the gene body were positively associated with gene expression. Integrated with the transcriptomic data, we found that all  cis -splicing genes were non-methylated, suggesting that DNA methylation may not associate with  cis -splicing. However, our results showed that 80% of  trans -splicing genes were methylated. Moreover, we found 1156 transcription factors (TFs) with reduced methylation and expression levels and 690 TFs with increased methylation and expression levels after drought treatment. These TFs may play important roles in  Populus  drought stress responses through the changes of DNA methylation.    Conclusions  These findings may provide valuable new insight into our understanding of the interaction between gene expression and methylation of drought responses in  Populus .

Single-base-resolution methylomes of populus trichocarpa reveal the association between DNA methylation and drought stress