Open AccessSimultaneous Detection of Mycobacterium tuberculosis and Atypical Mycobacteria by DNA-Microarray in Egypt
Author(s) -
Gaballah Ahmed,
Ghazal Abeer,
Almiry Reda,
Emad Rasha,
Sadek Nadia,
Abdel Rahman Mohamed,
El-Sherbini Eglal
Publication year - 2022
Publication title -
medical principles and practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.426
H-Index - 45
eISSN - 1423-0151
pISSN - 1011-7571
DOI - 10.1159/000524209
Subject(s) - original paper
Background and Objectives: Immunocompromised patients are a high-risk group for developing mycobacterial infections with either pulmonary and/or extrapulmonary diseases. Low-cost/density DNA-microarray is considered an easy and efficient method for the detection of typical and atypical mycobacterial species. Materials and Methods: Thirty immunocompromised patients were recruited to provide their clinical specimens (sputum, serum, urine, and lymph node aspirates). Real-time polymerase chain reaction (PCR) and DNA-microarray techniques were performed and compared to the conventional methods of Ziehl-Neelsen staining and Lowenstein Jensen culturing. Results: Mycobacterium tuberculosis complex was detected in all 30 clinical specimens (100% sensitivity) by real-time PCR and DNA-microarray. Additionally, coinfection with 4 atypical species belonging to nontuberculous mycobacteria was identified in 7 sputum specimens. These atypical mycobacterial species were identified as M. kansasii 10% ( n = 3), M. avium complex 6.6% ( n = 2), M. gordanae 3.3% ( n = 1), and M. peregrinum 3.3% ( n = 1). Conclusion: This study documents the presence of certain species of atypical mycobacteria among immunocompromised patients in Egypt.