Open AccessAntiarrhythmic Mechanisms of Chinese Herbal Medicine Dingji Fumai Decoction
Author(s) -
Bo Liang,
Yan Zhou,
Ling Fu,
Hui-Ling Liao
Publication year - 2020
Publication title -
evidence-based complementary and alternative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.552
H-Index - 90
eISSN - 1741-4288
pISSN - 1741-427X
DOI - 10.1155/2020/9185707
Subject(s) - aconitine , pharmacology , decoction , medicine , malondialdehyde , oxidative stress , superoxide dismutase , chemistry , traditional medicine
Background Dingji Fumai decoction (DFD) is used to treat ventricular arrhythmia, and it has provided a very good curative effect. However, its cellular electrophysiological mechanism is unknown.Methods Electrocardiogram was recorded, and oxidative stress response and ion-channel-related molecules were detected in rats with barium chloride- and aconitine-induced ventricular arrhythmia. Moreover, whole-cell patch-clamp assay was used to investigate the inhibitory effect of DFD on Nav 1.5 in Chinese hamster ovary cells.Results DFD prolonged the occurrence time and shortened the duration of ventricular arrhythmia, decreased the malondialdehyde and increased the superoxide dismutase, and alleviated the activation of Na + -K + -ATPase and connexin-43. DFD suppressed Nav 1.5 dose-dependently with an IC 50 of 24.0 ± 2.4 mg/mL.Conclusions The clinical antiarrhythmic mechanisms of DFD are based on its antioxidant potential, alleviation of Na + -K + -ATPase and connexin-43, and class I antiarrhythmic properties by suppressing Nav 1.5 dose-dependently with an IC 50 of 24.0 ± 2.4 mg/mL.